The directors of the NIH funded Digestive Disease Research Core Centers (DDRCC) are conducting a survey to examine the perceptions of how animal husbandry and experimental practices influence microbiome data variability. DDRCC In March 2018, representatives from six of the eighteen DDRCC gathered for their annual Midwest Alliance meeting to discuss the latest findings in […]
In a recent webinar, Dr. Alex Rodriguez-Palacios of Case Western Reserve University School of Medicine discussed the use of a new, low-cost caging system for conducting microbiome research in rodents. The speaker shared their experience with the NesTiso system and discussed: How NesTiso makes gnotobiotic research simple and practical How to inexpensively verify the germ-free […]
This book is the outcome of Workshop on Animal Models for Microbiome Research: Advancing Basic Science and Translational Research, hosted by the Institute for Laboratory Animal Research (ILAR). Microbiome and germ-free researchers James Fox, Joseph Newsome, Wendy Garrett, Jeffrey Gordon and Vincent Young organized the workshop, with the following goals: Improve the depth and breadth […]
On August 8, 2018, Randi Lundberg, DVM, PhD published a review paper titled, Humanizing the gut microbiota of mice: Opportunities and challenges in the journal, Laboratory Animals. The author acknowledges the need for in vivo models to study the human microbiome’s interaction with a biologically relevant host. Mice are one of the most popular animal […]
You may have missed these publications in the recent rush of microbiome and germ-free research news, but researchers are making interesting connections between the gut microbiome and neurological functioning. Four studies examined relationships between the gut microbiome and mood, behavior, disease risk, and development. Prenatal Stress, the Microbiome, and Development Scientists at Ohio State University’s […]
Heading off on vacation? Pack your beach bag with one of these 10 cutting-edge scientific books to keep your mind active this summer: Pandora’s Lab: Seven Stories of Science Gone Wrong, by Paul A. Offit MD The Hidden Half of Nature: The Microbial Roots of Life and Health, by David R. Montgomery and Anne Bikle […]
An increased interest in studies involving the microbiota requires animal models that appropriately mimic the extremely complex normal gut microbiota. Differences in rodent models can have a negative impact on a study’s reproducibility. On July 4, 2018, researchers at the University of Missouri, University of California, Davis, and University of North Carolina at Chapel Hill […]
While developing a novel gnotobiotic housing strategy, researchers on the Nested Isolation project discovered a cyclical bias in the microbiome composition of animals in SPF cages. These changes in microbiota could influence phenotype and confound study endpoints, with implications for the reproducibility of some gnotobiotic research. How can you best identify and control for cyclical […]
Scientists have found that deleting a single gene in a particular strain of gut bacteria causes changes in metabolism and reduced weight gain in mice, according to a study in the journal eLife. The research provides an important step towards understanding how the microbiome – the bacteria that live in our body – affects metabolism. […]
ABSTRACT Interactions between the gut microbial ecosystem and host lipid homeostasis are highly relevant to host physiology and metabolic diseases. We present a comprehensive multi-omics view of the effect of intestinal microbial colonization on hepatic lipid metabolism, integrating transcriptomic, proteomic, phosphoproteomic, and lipidomic analyses of liver and plasma samples from germfree and specific pathogen-free mice. […]
ABSTRACT Periodontal disease (PD) is a common dental disease associated with the interaction between dysbiotic oral microbiota and host immunity. It is a prevalent disease, resulting in loss of gingival tissue, periodontal ligament, cementum and alveolar bone. PD is a major form of tooth loss in the adult population. Experimental animal models have enabled the […]
ABSTRACT Gut microbiome plays an essential role in host health through host–gut microbiota metabolic interactions. Desirable modulation of beneficial gut bacteria, such as Akkermansia muciniphila, can confer health benefits by altering microbiome-related metabolic profiles. The purpose of this study is to examine the effects of a black raspberry-rich diet to reshape the gut microbiome by […]
SUMMARY Probiotics are widely prescribed for prevention of antibiotics-associated dysbiosis and related adverse effects. However, probiotic impact on post-antibiotic reconstitution of the gut mucosal host-microbiome niche remains elusive. We invasively examined the effects of multi-strain probiotics or autologous fecal microbiome transplantation (aFMT) on post-antibiotic reconstitution of the murine and human mucosal microbiome niche. Contrary to […]
SUMMARY Empiric probiotics are commonly consumed by healthy individuals as means of life quality improvement and disease prevention. However, evidence of probiotic gut mucosal colonization efficacy remains sparse and controversial. We metagenomically characterized the murine and human mucosal-associated gastrointestinal microbiome and found it to only partially correlate with stool microbiome. A sequential invasive multi-omics measurement […]
ABSTRACT Patients with Parkinson’s disease (PD) often have non-motor symptoms related to gastrointestinal (GI) dysfunction, such as constipation and delayed gastric emptying, which manifest prior to the motor symptoms of PD. Increasing evidence indicates that changes in the composition of the gut microbiota may be related to the pathogenesis of PD. However, it is unclear […]
ABSTRACT Isoorientin (ISO) is a natural flavonoid which is a 6-C-glucoside of luteolin and has been demonstrated to possess the multiple biological properties. In this study, the effects of ISO on the growth performance and gut microbiota of BALB/c mice were investigated. The results showed that ISO could promote food intake and body weight gain, […]
Rationale Growing evidence supports a role for the microbiota in regulating gut-brain interactions and, thus, psychiatric disorders. Despite substantial scientific efforts to delineate the mechanism of action of psychotropic medications at a central nervous system (CNS) level, there remains a critical lack of understanding on how these drugs might affect the microbiota and gut physiology. […]
Physiologic microbe-host interactions in the intestine require the maintenance of the microbiota in a luminal compartment through a complex interplay between epithelial and immune cells. However, the roles of mucosal myeloid cells in this process remain incompletely understood. In this study, we identified that decreased myeloid cell phagocytic activity promotes colon tumorigenesis. We show that […]
Significance Studies using experimental models have indicated that multiple sclerosis (MS)-like disease can be triggered in the gut following interactions of brain autoimmune T lymphocytes with local microbiota.
Gnotobiotics, edited by Trenton Schoeb and Kathryn Eaton, is invaluable to those developing or managing gnotobiotic facilities.
Brigham Young University: We all know that a poor diet is unhealthy, but a new BYU study finds that stress may just as harmful to our bodies as a really bad diet.
Abstract For more than three decades it has been recognized that small amounts of vector saliva can significantly alter the infectivity of vector-borne pathogens and subsequent in vivo dynamics.
Abstract Mouse models have enabled breakthroughs in our understanding of the immune system, but it has become increasingly popular to emphasize their shortcomings when translating observations to humans.
Highlights •Systematic analysis of longevity-promoting microbial genetic variations •Colanic acid as a pro-longevity natural compound effective in different species