In order to maintain germ-free colonies, researchers must implement specialized techniques and husbandry requirements not needed in standard vivariums. This is critical to prevent contamination within a gnotobiotic facility. Download this webinar to learn about appropriate validation of supply sterilization and recommended monitoring techniques for germ-free and gnotobiotic isolators. By watching you can learn: Autoclave, […]
Dr. Cristina Weiner recently presented a webinar featuring a novel approach to germ-free derivations. She discussed the importance of understanding baseline performance and making incremental process improvements as well as how Taconic Biosciences developed and implemented a new facility design which supports improved germ-free derivation success. Q: You stated during the presentation that, on balance, […]
The microbiome needs no introduction – it has been several years since you could pick up a biomedical research journal and not run into an article on possible connections of human gut bacteria and disease. There were thousands of such papers last year alone. But it’s a very hard field to work in. You can […]
Download this webinar to learn a novel approach to germ-free derivations outside of the limitations of an isolator — an approach focused on control of the macroenvironment. Dr. Cristina Weiner, VMD, MS, DACLAM, presents and shares how applying cleanroom technology to gnotobiotic animal husbandry has resulted in the improved success of germ-free derivations. By watching […]
Are you looking for a microbiome conference or workshop? Many 2020 conferences are outlined on our event’s page with differing agendas and meetings in the U.S., Europe, and Asia, these educational summits provide an opportunity to network and meet your peers. Here are the first few microbiome conferences of this year: Rhode Island Microbiome Symposium […]
Dr. Philip Dubé recently presented a webinar on validated Il10 knockout mouse models for IBD research. He gave an overview of research in this area and presented new data on these models from studies done by Boehringer Ingelheim and Incyte. Due to time constraints, many of the questions submitted during the webinar went unanswered. This […]
As microbiome science expands, academic centres scramble to fill many needs, from service provider to industry liaison. A newly created network aims to share strategies and accelerate knowledge transfer, and invites others to join the efforts. Microbiomes — assemblages of interacting microorganisms, such as bacteria, archaea, fungi and viruses — are increasingly recognized as being […]
Jonathan Eisen is compiling a list of Microbiome Centers from the US and elsewhere. Jonathan had previously done this and have no made a new effort in part in association with a meeting of Microbiome Centers organized by Jen Hughes Martiny and others of the UC Irvine Microbiome Initiative. He would love suggestions for other […]
The microbiome continues to be an important topic in both human health research and in popular culture. The microbiome is cited as a factor in multiple diseases, neurological conditions, and immune responses to foreign pathogens. Specific compositions of gut commensal bacteria have been associated with an increased incidence of depression, varying responses to immunotherapies, and […]
Abstract Background Roux-en-Y gastric bypass (RYGB) surgery is a last-resort treatment to induce substantial and sustained weight loss in cases of severe obesity. This anatomical rearrangement affects the intestinal microbiota, but so far, little information is available on how it interferes with microbial functionality and microbial-host interactions independently of weight loss. Methods A rat model […]
ABSTRACT Background Fasting and timed feeding strategies normalize obesity parameters even under high-fat dietary intake. Although previous work demonstrated that these dietary strategies reduce adiposity and improve metabolic health, limited work has examined intestinal microbial communities. Objectives We determined whether timed feeding modifies the composition of the intestinal microbiome and mycobiome (yeast and fungi). Methods […]
Abstract Background and aim PI3Kγ is closely related to inflammation and cardiovascular diseases and thus, PI3Kγ inhibitors are candidate drugs for the treatment of these disorders. Considering the potential effect of the intestinal microbiome on inflammation and cardiovascular diseases, this study aimed to identify characteristics of the intestinal microbial community under PI3Kγ deficiency, to help […]
ABSTRACT Glufosinate ammonium (GLA) is a widely used organophosphate herbicide, which could be commonly detected in body fluids of both pregnant women and newborns. Existing evidences indicate that GLA has reproductive toxicity, while data concerning the effects of prenatal GLA exposure on neurodevelopment is rather limited. Here we employed a mouse model exposed to GLA […]
Abstract Background The gut microbiota is closely associated with the bidirectional gut-brain axis that modulates neuropsychological functions of the central nervous system, thereby affecting mental disorders such as depression. Although it is known that probiotics affect brain functions, the impact of probiotics on the regulation of the prevalence and composition of gut microbiota, leading to […]
ABSTRACT Human diseases are increasingly linked with an altered or “dysbiotic” gut microbiota, but whether such changes are causal, consequential, or bystanders to disease is, for the most part, unresolved. Human microbiota-associated (HMA) rodents have become a cornerstone of microbiome science for addressing causal relationships between altered microbiomes and host pathology. In a systematic review, […]
ABSTRACT Early life changes in the microbiome contribute to the development of allergic asthma, but little is known about the importance of the microbiome for other forms of asthma. Ozone is a nonatopic asthma trigger that causes airway hyperresponsiveness and neutrophil recruitment to the lungs. The purpose of this study was to test the hypothesis […]
Hidden benefits of a fecal transplant Our gut microbiota evolves as we age, yet its effects on host physiology are not clearly understood. Kundu et al. now attempt to elucidate these effects by transplanting the gut microbiota of either young or old donor mice into young germ-free recipient mice. They report that young germ-free mice […]
Abstract We investigated the effect of a combination treatment with dapagliflozin (Dapa), a sodium-glucose cotransporter-2 inhibitor and butyrate on weight change in db/db mice. Six-week-old male db/db mice were assigned to four groups: vehicle with normal chow diet (NCD), Dapa with NCD, vehicle with 5% sodium butyrate-supplemented NCD (NaB), or Dapa with 5% NaB. After […]
Significance Studies using experimental models have indicated that multiple sclerosis (MS)-like disease can be triggered in the gut following interactions of brain autoimmune T lymphocytes with local microbiota.
Gnotobiotics, edited by Trenton Schoeb and Kathryn Eaton, is invaluable to those developing or managing gnotobiotic facilities.
Brigham Young University: We all know that a poor diet is unhealthy, but a new BYU study finds that stress may just as harmful to our bodies as a really bad diet.
Abstract For more than three decades it has been recognized that small amounts of vector saliva can significantly alter the infectivity of vector-borne pathogens and subsequent in vivo dynamics.
Abstract Mouse models have enabled breakthroughs in our understanding of the immune system, but it has become increasingly popular to emphasize their shortcomings when translating observations to humans.
Highlights •Systematic analysis of longevity-promoting microbial genetic variations •Colanic acid as a pro-longevity natural compound effective in different species