Short-term microbiome studies might not require isolators

Posted by: | September 16, 2014 | Comments


Submitted By: Dr. Martin Toft, Taconic Biosciences:

It seems it just got easier to do microbiome studies in rodents – at least short term experiments. In the September issue of Laboratory animals, Hecht el al (Link 1) reported the successful germ-free housing of mice in individually ventilated cages (IVC) for up to 3 weeks. The system used is the Isocage from Tecniplast (Link 2), which is a high level biocontainment/bioexclusion version of the traditional IVC-technology. Based on the results, the housing methodology proved capable of providing axenic conditions while placed in the rack, but the handling of cages and animals obviously is a much more sensitive process when performed in a hood. Nevertheless, proper aseptic cage-changing procedures can be performed and the findings prove that alternatives to isolator housing are available when performing short-term studies requiring germ-free conditions.

Significant additional benefits include increased flexibility and decreased cost. It also means that facilities without isolator capabilities can now consider microbiome experimentation in rodents as germ-free animals! Resources to support this effort can be obtained from external sources; see Link 3.

Link 1: Link to abstract: Sage
Link 2: Link to be posted on the Resources-Services page: tecniplast
Link 3: Link Resources-Services page Translational Microbiome

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