Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

Posted by: | June 14, 2017 | Comments

helicobacter-pylori-s3-cause

Abstract

In this chapter, we describe a fluorescence in vivo hybridization (FIVH) protocol, using nucleic acid probes, for the detection of the bacterium Helicobacter pylori in the gastric mucosa of an infected C57BL/6 mouse model. This protocol should be easily extended to other microorganisms not only as a way to identify in vivo important microorganisms and their patterns of distribution within specific or at different anatomic sites, but also to better understand interaction mechanisms involving the microbiome and the human body.

Learn More at: Springer Link

Silvia Fontenete, Marina Leite, Ceu Figueiredo, Paul Cos, Nuno F. Azevedo. Diagnostic Bacteriology. Volume 1616 of the series Methods in Molecular Biology pp 137-146. Date: 10 June 2017. DOI 10.1007/978-1-4939-7037-7_8. Print ISBN 978-1-4939-7035-3. Online ISBN 978-1-4939-7037-7. Series Title Methods in Molecular Biology. Series Volume 1616. Series ISSN 1064-3745.





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