Isoorientin (ISO) is a natural flavonoid which is a 6-C-glucoside of luteolin and has been demonstrated to possess the multiple biological properties. In this study, the effects of ISO on the growth performance and gut microbiota of BALB/c mice were investigated. The results showed that ISO could promote food intake and body weight gain, increase the digestibility of crude proteins and utilization of the gross energy, and strengthen antioxidant capacity of mice. We also demonstrated it has no side-effects on the hepatic and renal function. Moreover, ISO inhibited growth of the most bacteria in gut microbiota, especially the pathogenic genera of Alistipes, Helicobacter and Oscillibacter which could lead to inflammation. Metabolisms of epithelial cell signaling in Helicobacter pylori infection, Lipopolysaccharide (LPS) biosynthesis, and LPS biosynthesis proteins in gut microbiota of control group were more abundant than that in ISO group, while lipid metabolism and Vitamin B6 metabolism were enriched in ISO group. We found the changes in enrichments of metabolic pathways of the gut microbiota along with the ISO application were positively correlated with the anti-oxidation, anti-inflammation, and antibiosis. This work provided a fundamental basis for the future development of ISO-functional foods used for resistance to oxidation, inflammation, and pathogens.
Read more at: ACS Publications