Hybridization capture reveals microbial diversity missed using current profiling methods

Posted by: | March 28, 2018 | Comments

ABSTRACT

Microorganisms comprise the majority of living organisms on our planet. For many years, exploration of the composition of microbial communities has been performed through the PCR-based study of the small subunit rRNA gene due to its high conservation across the domains of life. The application of this method has resulted in the discovery of many unexpected evolutionary lineages. However, amplicon sequencing is subject to numerous biases, with some taxa being missed, and is limited by the read length of second-generation sequencing platforms, which drastically reduces the phylogenetic resolution.

Read more at: SpringerLink

Cyrielle Gasc, Pierre Peyret. SpringerLink. DOI: https://doi.org/10.1186/s40168-018-0442-3. 27 March 2018.





Stay up-to-date!
Email Address *
First Name
Last Name

* indicates required
Privacy Policy




Terms & Conditions | Privacy Policy | © 2018 The Translational Microbiome Research Forum