Nod2 Deficiency in mice is Associated with Microbiota Variation Favouring the Expansion of mucosal CD4+ LAP+ Regulatory Cells

Posted by: | October 5, 2018 | Comments

Experimental design on Nod2−/− and Nod2+/+ mice to investigate the effect of genetic background and housing conditions on microbiota-immune-system interactions.

ABSTRACT

Nucleotide-binding Oligomerization Domain-2 (NOD2) mutations are associated with an increased risk to develop Crohn’s Disease. In previous studies, we have shown that Nod2−/− mice manifest increased proportion of Lamina Propria (LP) CD4+ LAP+ Foxp3− regulatory cells, when compared with Nod2+/+ mice, while CD4+ Foxp3 + regulatory cells were not affected. Here, we investigated the Nod2 gut microbiota, by 16S rRNA pyrosequencing, at steady state and after TNBS-colitis induction in mice reared separately or in cohousing, correlating the microbial profiles with LP regulatory T cells proportion and tissue cytokines content. We found that enrichment of Rikenella and Alistipes (Rikenellaceae) in Nod2−/− mice at 8 weeks of age reared separately was associated with increased proportion of CD4+ LAP+ Foxp3− cells and less severe TNBS-colitis.

Read more at: Scientific Reports

A. Butera, M. Di Paola, L. Pavarini, F. Strati, M. Pindo, M. Sanchez, D. Cavalieri, M. Boirivant & C. De Filippo. Scientific Reports. DOI: 10.1038/s41598-018-32583-zID. 24 September 2018.





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