Replication Study: Intestinal inflammation targets cancer-inducing activity of the microbiota

Posted by: | October 15, 2018 | Comments

Detection of pks island and impact of pks island deletion on bacterial growth in vitro.


As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Eaton et al., 2015) that described how we intended to replicate selected experiments from the paper “Intestinal Inflammation Targets Cancer-Inducing Activity of the Microbiota” (Arthur et al., 2012). Here we report the results. We observed no impact on bacterial growth or colonization capacity when the polyketide synthase (pks) genotoxic island was deleted from E. coli NC101, similar to the original study (Supplementary Figure 7; Arthur et al., 2012). However, for the experiment that compared inflammation, invasion, and neoplasia in azoxymethane (AOM)-treated interleukin-10-deficient mice mono-associated with NC101 or NC101Δ pks the experimental timing of the replication attempt was longer than that of the original study.

Read more at: eLife

Kathryn Eaton, et al. eLife. DOI: 10.7554/eLife.34364. 2018.

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