The influence of caging, bedding, and diet on the composition of the microbiota in different regions of the mouse gut

Posted by: | March 12, 2018 | Comments

The influence of caging, bedding, and diet on the composition of the microbiota in different regions of the mouse gut

ABSTRACT

Countless studies have identified differences between the gut microbiota of humans affected with myriad conditions and healthy individuals, and animal models are commonly used to determine whether those differences are causative or correlative. Recently, concerns have arisen regarding the reproducibility of animal models between institutions and across time. To determine the influence of three common husbandry-associated factors that vary between institutions, groups of weanling mice were placed in either static or ventilated microisolator caging, with either aspen or paperchip bedding, and with one of three commonly used rodent chows, in a fully crossed study design. After thirteen weeks, samples were collected from multiple regions of the gastrointestinal tract and characterized using culture-independent sequencing methods.

Read more at: Scientific Reports

Aaron C. Ericsson, Jonalyn Gagliardi, Delia Bouhan, William G. Spollen, Scott A. Givan & Craig L. Franklin. DOI: 10.1038/s41598-018-21986-7. 6 March 2018.





Stay up-to-date!
Email Address *
First Name
Last Name

* indicates required
Privacy Policy




Terms & Conditions | Privacy Policy | © 2018 The Translational Microbiome Research Forum