Harnessing the Power of PCR Molecular Fingerprinting Methods and Next Generation Sequencing for Understanding Structure and Function in Microbial Communities

Posted by: | June 2, 2023 | Comments


Polymerase chain reaction (PCR) is central to methods in molecular ecology. Here, we describe PCR-dependent approaches useful for investigating microbial diversity and its function in various natural, human-associated, and built environment ecosystems. Protocols routinely used for DNA extraction, purification, cloning, and sequencing are included along with various resources for the statistical analysis following gel electrophoresis-based methods (DGGE) and sequencing. We also provide insights into eukaryotic microbiome analysis, sample preservation techniques, PCR troubleshooting, DNA quantification methods, and commonly used ordination techniques.

Discover More at: Springer Link

Sujal Phadke , Andreia Filipa Salvador , Joana Isabel Alves , Orianna Bretschger, Maria Madalena Alves, Maria Alcina Pereira. Volume 1620 of the series Methods in Molecular Biology pp 225-248. DOI 10.1007/978-1-4939-7060-5_16. Print ISBN 978-1-4939-7059-9. Online ISBN 978-1-4939-7060-5.

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