A Humanized Diet Profile May Facilitate Colonization and Immune Stimulation in Human Microbiota-Colonized Mice

Posted by: | July 21, 2020 | Comments

Study design. Timeline showing the six experimental groups. Germ-free parent (P) mice were colonized with human microbiota (HM) or mouse microbiota (MM) inoculum when 6 weeks old and received animal source (AS) diet, human profile (HP) diet or control (C) diet from the day of arrival in our facility.

Background:

In spite of the importance of the use of gnotobiotic mice for human fecal transfer, colonization efficiency and immune stimulation after human microbiota inoculation in mice are poorly studied compared to mouse microbiota inoculation. We tested the colonization efficiency and immune responses in mice bred for one additional generation after inoculating the parent generation with either a human (HM) or a mouse microbiota (MM). Furthermore, we tested if colonization efficiency and immune stimulation could be improved in HM-colonized mice by dietary approaches: if these were fed a diet closer to the human diet either in its sources of animal fat and protein [the “animal source” (AS) diet] or in its proportions of macronutrients from the normal sources of a mouse diet [the “human profile” (HP) diet].

Read more at: Frontiers

Isabel Moreno-Indias, Randi Lundberg, Lukasz Krych, Stine Broeng Metzdorff, Witold Kot, Dorte Bratbo Sørensen, Dennis Sandris Nielsen, Camilla Hartmann Friis Hansen, and Axel K. Hansen. Front Microbiol. DOI: https://doi.org/10.3389/fmicb.2020.01336. 19 June 2020.





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