Absorptive transport of amino acids by the rat colon

Posted by: | December 2, 2019 | Comments


Absorptive transport of amino acids by the rat colon

The capacity of the colon to absorb microbially-produced amino acids (AAs) and the underlying mechanisms of AA transport are incompletely defined. We measured the profile of 16 fecal AAs along the rat ceco-colonic axis and compared unidirectional absorptive AA fluxes across mucosal tissues isolated from the rat jejunum, cecum, and proximal colon using an Ussing chamber approach in conjunction with 1H-NMR and UPLC-MS chemical analyses. Passage of stool from cecum to mid-colon was associated with segment-specific changes in fecal AA composition and a decrease in total AA content. Simultaneous measurement of up to 16 AA fluxes under native luminal conditions, with correction for endogenous AA release, demonstrated absorptive transfer of AAs across the cecum and proximal colon at rates comparable (30-80%) to those across the jejunum, with significant Na+-dependent and H+-stimulated components.

Read more at: Gastrointestinal and Liver Physiology

Yuxin Chen, Meredith M. Dinges, Andrew Green, Scott E. Cramer, Cynthia K. Larive, and Christian Lytle. American Journal of Physiology-Gastrointestinal and Liver Physiology. DOI: https://doi.org/10.1152/ajpgi.00277.2019. 25 November 2019.

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