Degeneration of the intestinal microbial community in PI3Kγ-knockout mice

Posted by: | February 14, 2020 | Comments

Abstract

Background and aim

Degeneration of the intestinal microbial community in PI3Kγ-knockout mice

PI3Kγ is closely related to inflammation and cardiovascular diseases and thus, PI3Kγ inhibitors are candidate drugs for the treatment of these disorders. Considering the potential effect of the intestinal microbiome on inflammation and cardiovascular diseases, this study aimed to identify characteristics of the intestinal microbial community under PI3Kγ deficiency, to help reveal the potential influence of PI3Kγ inhibitors mediated by the microbial community.

Methods

Exon 2 of the PI3Kγ gene was knocked out in a Balb/c mouse by using single-guide RNAs. Homozygous PI3Kγ-knockout (PI3Kγ–/–) mice were obtained by embryo transfer and hybridization. PI3Kγ–/– and wild-type (WT) mice were raised in the same specific pathogen-free conditions until 8 weeks of age. Then, colonic tissues and feces from the middle segment of the colon were collected and analyzed by Illumina MiSeq sequencing.

Read more at: ScienceDirect

Yi Li, Qian-Qian Chen, Jian Yuan, Hai-Xu Chen, Jun Wan. Microbial Pathogenesis. DOI: https://doi.org/10.1016/j.micpath.2020.104038. 03 February 2020.





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