Depletion of microbiome-derived molecules in the host using Clostridium genetics

Posted by: | December 18, 2019 | Comments

Clostridial metabolite production

Depletion of microbiome-derived molecules in the host using Clostridium genetics

The clostridia are Firmicute bacterial commensals commonly found in the mammalian gut. Clostridia produce a range of metabolites that diffuse into the host’s circulation and have been difficult to manipulate genetically, but Guo et al. successfully developed a CRISPR-Cas9 deletion system in Clostridium sporogenes (see the Perspective by Henke and Clardy). The authors used deletion mutants and mass spectrometry to elucidate clostridial synthesis of several different branched short-chain fatty acids (SCFAs), including isobutyrate, 2-methylbutyrate, and isovalerate. Germ-free mice colonized with mutants incapable of synthesizing SCFAs showed altered immunoglobulin A production. This finding potentially links bacterial SCFA production and host responses to the presence of the clostridia.

Read more at: Science

Chun-Jun Guo, Breanna M. Allen, Kamir J. Hiam, Dylan Dodd, Will Van Treuren, Steven Higginbottom, Kazuki Nagashima, Curt R. Fischer, Justin L. Sonnenburg, Matthew H. Spitzer, and Michael A. Fischbach. Science. DOI: 10.1126/science.aav1282. 13 December 2019.





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