Developing standards for the microbiome field

Posted by: | July 27, 2020 | Comments

Visualisation of the relationship between different mock communities following sequencing and taxonomic profiling by a variety of approaches. A nMDS plot of a Bray-Curtis dissimilarity matrix was constructed from the species composition of five reference reagents following shotgun sequencing and taxonomic profiling by five different bioinformatics tools, MetaPhlAn2, Kaiju, Kraken, Bracken, and Centrifuge. Gut-HiLo = NIBSC Gut-HiLo-RR. Gut-Mix = NIBSC Gut-Mix-RR. MSA_1000 = ATCC MSA-1000. MSA_1001 = ATCC MSA-1001. MSA_1002 = ATCC MSA-1002. MSA_1003 = ATCC MSA-1003. Zymo = ZymoBIOMICS Microbial Community Standard

Abstract

Background

Effective standardisation of methodologies to analyse the microbiome is essential to the entire microbiome community. Despite the microbiome field being established for over a decade, there are no accredited or certified reference materials available to the wider community. In this study, we describe the development of the first reference reagents produced by the National Institute for Biological Standards and Control (NIBSC) for microbiome analysis by next-generation sequencing. These can act as global working standards and will be evaluated as candidate World Health Organization International Reference Reagents.

Read more at: Microbiome

Gregory C. A. Amos, Alastair Logan, Saba Anwar, Martin Fritzsche, Ryan Mate, Thomas Bleazard & Sjoerd Rijpkema. Microbiome. DOI: https://doi.org/10.1186/s40168-020-00856-3. 26 June 2020.





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