Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis

Posted by: | November 12, 2023 | Comments


Intestinal mucus layer disruption and gut microflora modification in conjunction with tight junction (TJ) changes can increase colonic permeability that allows bacterial dissemination and intestinal and systemic disease. We showed previously that Citrobacter rodentium (CR)-induced colonic crypt hyperplasia and/or colitis is regulated by a functional cross-talk between the Notch and Wnt/β-catenin pathways. In the current study, mucus analysis in the colons of CR-infected (108 CFUs) and Notch blocker Dibenzazepine (DBZ, i.p.; 10μmol/Kg b.w.)-treated mice revealed significant alterations in the composition of trace O-glycans and complex type and hybrid N-glycans, compared to CR-infected mice alone that preceded/accompanied alterations in 16S rDNA microbial community structure and elevated EUB338 staining. While mucin-degrading bacterium, Akkermansia muciniphila (A. muciniphila) along with Enterobacteriaceae belonging to Proteobacteria phyla increased in the feces, antimicrobial peptides Angiogenin-4, Intelectin-1 and Intelectin-2, and ISC marker Dclk1, exhibited dramatic decreases in the colons of CR-infected/DBZ-treated mice.

Read more at: PLoS ONE

Ishfaq Ahmed, Badal C. Roy, Rita-Marie T. Raach, Sarah M. Owens, Lijun Xia, Shrikant Anant, Venkatesh Sampath, and Shahid Umar. PLoS ONE. DOI: https://doi.org/10.1371/journal.pone.0206701. November 1, 2023.

Stay up-to-date!
Email Address *
First Name
Last Name

* indicates required
Privacy Policy

Terms & Conditions | Privacy Policy | © 2021 The Translational Microbiome Research Forum

As of 3/31 the forum will no longer be active. Please visit taconic.com/events for future bioscience events as Taconic remains committed to leading the industry in microbiome solutions. Thank you for your support.