Gastric bypass surgery in a rat model alters the community structure and functional composition of the intestinal microbiota independently of weight loss

Posted by: | February 19, 2020 | Comments

Abstract

Background

Experimental setup with anatomy of the gut after RYGB: analysis methods used on samples

Roux-en-Y gastric bypass (RYGB) surgery is a last-resort treatment to induce substantial and sustained weight loss in cases of severe obesity. This anatomical rearrangement affects the intestinal microbiota, but so far, little information is available on how it interferes with microbial functionality and microbial-host interactions independently of weight loss.

Methods

A rat model was employed where the RYGB-surgery cohort is compared to sham-operated controls which were kept at a matched body weight by food restriction. We investigated the microbial taxonomy and functional activity using 16S rRNA amplicon gene sequencing, metaproteomics, and metabolomics on samples collected from theileum, the cecum, and the colon, and separately analysed the lumen and mucus-associated microbiota.

Results

Altered gut architecture in RYGB increased the relative occurrence of Actinobacteria, especially Bifidobacteriaceae and Proteobacteria, while in general, Firmicutes were decreased although Streptococcaceae and Clostridium perfringens were observed at relative higher abundances independent of weight loss.

Read more at: Microbiome

Sven-Bastiaan Haange, Nico Jehmlich, Ute Krügel, Constantin Hintschich, Dorothee Wehrmann, Mohammed Hankir, Florian Seyfried, Jean Froment, Thomas Hübschmann, Susann Müller, Dirk K. Wissenbach, Kang Kang, Christian Buettner, Gianni Panagiotou, Matthias Noll, Ulrike Rolle-Kampczyk, Wiebke Fenske & Martin von Bergen. Microbiome. DOI: https://doi.org/10.1186/s40168-020-0788-1. 07 February 2020.





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