Generation of orthotopically functional salivary gland from embryonic stem cells

Posted by: | November 26, 2023 | Comments

ABSTRACT

Organoids generated from pluripotent stem cells are used in the development of organ replacement regenerative therapy by recapitulating the process of organogenesis. These processes are strictly regulated by morphogen signalling and transcriptional networks. However, the precise transcription factors involved in the organogenesis of exocrine glands, including salivary glands, remain unknown. Here, we identify a specific combination of two transcription factors (Sox9 and Foxc1) responsible for the differentiation of mouse embryonic stem cell-derived oral ectoderm into the salivary gland rudiment in an organoid culture system. Following orthotopic transplantation into mice whose salivary glands had been removed, the induced salivary gland rudiment not only showed a similar morphology and gene expression profile to those of the embryonic salivary gland rudiment of normal mice but also exhibited characteristics of mature salivary glands, including saliva secretion. This study suggests that exocrine glands can be induced from pluripotent stem cells for organ replacement regenerative therapy.

Read more at: Nature

Junichi Tanaka, et al. Nature Communications. https://doi.org/10.1038/s41467-018-06469-7. October 11, 2023.





Stay up-to-date!
Email Address *
First Name
Last Name

* indicates required
Privacy Policy




Terms & Conditions | Privacy Policy | © 2021 The Translational Microbiome Research Forum

As of 3/31 the forum will no longer be active. Please visit taconic.com/events for future bioscience events as Taconic remains committed to leading the industry in microbiome solutions. Thank you for your support.