Long‐term feeding of a high‐fat diet (HFD) causes gastrointestinal inflammation and gut microbiota disturbance, leading to the increased occurrence of obesity and anxiety. In the present study, we examined the effects of heat‐labile Lactobacillus sakei OK67, tyndallized OK67 (tOK67), and heat‐stable Lactobacillus sakei PK16 on HFD‐induced obesity and anxiety in mice.
Methods and results
Obesity was induced in mice by feeding with HFD. Oral administration of live OK67, tOK67, or PK16 reduced HFD‐induced body and liver weights and blood triglyceride, total cholesterol, corticosterone, and lipopolysaccharide levels. These treatments also suppressed HFD‐induced NF‐κB activation and increased HFD‐suppressed AMP‐activated protein kinase (AMPK) activation and SIRT‐1 expression in the liver. OK67 or PK16 treatment alleviated HFD‐induced anxiety‐like behaviors and increased BDNF expression and NF‐κB activation in the hippocampus. Moreover, OK67 or PK16 treatment suppressed HFD‐induced colitis and suppressed the Proteobacteria population and fecal lipopolysaccharide levels in mice. OK67 or PK16 treatment inhibited NF‐κB activation and induced AMPK activation and SIRT‐1 expression in lipopolysaccharide‐stimulated Caco‐2 cells. Overall, the anti‐obesity and anxiolytic effects of live OK67 were more potent than those of tOK67.
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