Self-reinoculation with fecal flora changes microbiota density and composition leading to an altered bile-acid profile in the mouse small intestine

Posted by: | March 4, 2024 | Comments

An overview of the study design and timeline: Mice from two age cohorts (4-month-old and 8-month-old) were raised co-housed (four mice to a cage) for 2–6 months. One mouse from each cage was then assigned to one of the four experimental conditions: functional tail cups (TC-F), mock tail cups (TC-M), housing on wire floors (WF), and controls housed in standard conditions (CTRL). All mice were singly housed and maintained on each treatment for 12–20 days (N = 24, 6 mice per group).



The upper gastrointestinal tract plays a prominent role in human physiology as the primary site for enzymatic digestion and nutrient absorption, immune sampling, and drug uptake. Alterations to the small intestine microbiome have been implicated in various human diseases, such as non-alcoholic steatohepatitis and inflammatory bowel conditions. Yet, the physiological and functional roles of the small intestine microbiota in humans remain poorly characterized because of the complexities associated with its sampling. Rodent models are used extensively in microbiome research and enable the spatial, temporal, compositional, and functional interrogation of the gastrointestinal microbiota and its effects on the host physiology and disease phenotype.

Read more at: Microbiome

Said R. Bogatyrev, Justin C. Rolando & Rustem F. Ismagilov. Microbiome. DOI: 12 Feb 2024.

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